Analysis of Differential Display RT-PCR Products Using Fluorescent Primers and GENESCANTM Software
نویسندگان
چکیده
منابع مشابه
Analysis of differential display RT-PCR products using fluorescent primers and GENESCAN software.
Differential display reverse transcription PCR (DDRT-PCR) is a procedure used to identify the induction or repression of gene expression. In most DDRT-PCR protocols, radioisotopes are incorporated during PCR and the cDNA products are detected by autoradiography. This report describes the fluorescent labeling of cDNAs and their detection on automated sequencers from PE Applied Biosystems. A fluo...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1997
ISSN: 0736-6205,1940-9818
DOI: 10.2144/97221pf01